As in many areas of healthcare delivery, ambulatory care practice is being transformed by technological advances such as artificial intelligence (AI). Registered nurses (RNs) are challenged to integrate these new technologies into practice. As RNs are integral to care delivery, they must be knowledgeable about AI. In addition to nursing practice, nursing education will need to identify the knowledge, skills, and competencies required to practice with AI. In this study, the knowledge and attitudes of nursing students, practicing nurses, and nursing faculty about AI in nursing and health care were assessed.